Figure 1

Panel A: Whole blood glucose levels during an oral glucose tolerance test in Chow ( n = 5-7), HSD ( n = 5), and HFD ( n = 5) fed rats. Rats were food restricted by providing 4 g/rat of their respective diets at 5 PM the night prior to the OGTT. At 8 am the following morning, rats were administered an oral bolus of glucose (1 g/kg D-glucose) by gavage immediately following the initial glucose sample (0 min). Glucose levels returned to baseline by 120 mins in the Chow and HFD fed rats but remained elevated in HSD fed rats. Data expressed as means ± SEM. * p < 0.05 vs. corresponding time point in Chows; #p < 0.05 vs. initial blood glucose level (time 0); §p < 0.05 vs. corresponding level at 60 mins. Panel B: Measurement of oxidative stress in isolated mesenteric resistance arterioles from rats following the 6-week feeding protocol. 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate, acetyl ester (DCF) fluorescence (average gray scale values above background) in isolated mesenteric resistance arteries from Chow (n = 6), HSD (n = 5) and HFD (n = 5) fed rats. Data are expressed as means ± SEM. * p ≤ 0.05 vs. Chow fed controls