Table 1 Gene-specific primer sequences used in real-time PCR amplification

Gene	Primer sequence (5' → 3')	Product length (bp)	Primer efficiency
Adiponectin	F: GCTCAGGATGCTACTGTTG	255	1.9
	R: TCTCACCCTTAGGACCAAG
Leptin	F: TTGTCACCAGGATCAATGACATTT	106	1.9
	R: GACAAACTCAGAATGGGGTGAAG
MCP1	F: GCTCTCTCTTCCTCCACCAC	208	1.8
	R: GCTTCTTTGGGACACCTGCT
Emr1	F: TTTCCTCGCCTGCTTCTTC	222	1.8
	R: CCCCGTCTCTGTATTCAACC
IL-6	F: TGGGAAATCGTGGAAATGAG	249	1.9
	R: GAAGGACTCTGGCTTTGTCTT
TNFα	F: CGTCGTAGCAAACCACCAA	145	1.7
	R: GAGAACCTGGGAGTAGACAAGG
iNOS	F: GGCAGCTACTGGGTCAAAGA	172	1.8
	R: TCTGAGGGCTGACACAAGG
18S	F: CGCGGTTCTATTTTGTTGGT	219	1.9
	R: AGTCGGCATCGTTTATGGTC

F, forward; R, reverse. Target genes: adiponectin; leptin; monocyte chemotactic protein-1 (MCP1); epidermal growth factor module-containing mucin-like receptor 1 (Emr1); interleukin-6 (IL-6); tumor necrosis factor alpha (TNFα); inducible nitric oxide synthase (iNOS). 18S rRNA was used for normalization.

ISSN: 1743-7075