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Figure 1 | Nutrition & Metabolism

Figure 1

From: Identification of the Eph receptor pathway as a novel target for eicosapentaenoic acid (EPA) modification of gene expression in human colon adenocarcinoma cells (HT-29)

Figure 1

EPA induced changes in the expression of EphB4, Vav2 and EphA1. EPA induced changes in the expression of EphB4, Vav2 and EphA1 in HT29 cells as determined by microarray and real-time RT-PCR (TaqMan) over a 24 hr time course in two separate experiments. Data are expressed as log fold change relative to untreated control cells at the same time point. All data is normalized to the initial sample taken immediately after changing the media (< 20 min.). Open circles denote microarray data and filled triangles denote real-time RT-PCR TaqMan data. Each data point depicts the mean of 2-4 flasks of cells. The error bars represent standard deviation with significant difference relative to untreated cells (p < 0.05) represented by * for TaqMan data and ‡ for microarray data. (A) EphB4 is up-regulated in response to EPA treatment at 8 and 24 hr by more than 2 fold. B) Vav2 is up-regulated in response to EPA treatment as determined by real-time RT-PCR analysis at 24 hr by both microarray analysis (p < 0.05) and real-time RT-PCR (p < 0.02 - relative to expression between 1-6 hr). C) EphA1 is up-regulated at 8 and 24 hr in the first experiment using microarray analysis but not in the second experiment using real-time RT-PCR.

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Nutrition & Metabolism

ISSN: 1743-7075

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