Effects of diet-induced obesity and voluntary wheel running on the microstructure of the murine distal femur

Nutrition & Metabolism

Table 3 Trabecular parameters measured by μCT from the distal metaphysis of mouse femur dissected after 21-week intervention.

μCT	Control diet		High-fat diet		ANOVA (p value)
	C (n = 8)	CR (n = 9)	HF (n = 7)	HFR (n = 7)	Diet	Running	*DietRunning**
Conn.D (mm^-3)	337 (129)	367 (105)	466 (138)	415 (88)	<0.05	0.810	0.339
BV (10^-1 mm³)	0.093 (0.015)	0.103 (0.035)	0.112 (0.027)	0.146 (0.034)^ab	<0.01	0.069	0.333
BS (mm²)	1.59 (0.24)	1.70 (0.52)	1.85 (0.39)	2.23 (0.37)^ab	<0.05	0.106	0.363
BS/BV (mm^-1)	171 (6)	167 (10)	160 (10)	154 (11)^ab	<0.05	0.143	0.741
Tb.Pf (mm^-1)	72.8 (3.4)	69.2 (6.1)	72.1 (7.0)	63.9 (6.2)^ac	0.165	<0.01	0.260
SMI	2.60 (0.07)	2.54 (0.11)	2.74 (0.12)^b	2.53 (0.11)^c	0.089	<0.01	0.061
Tb.Th (μm)	23.7 (1.2)	23.7 (1.2)	26.1 (0.9)^ab	25.3 (1.7)^ab	<0.01	0.446	0.388
Tb.N (mm^-1)	0.288 (0.020)	0.330 (0.112)	0.339 (0.078)	0.429 (0.090)^ab	0.068	0.158	0.290
Tb.Sp (μm)	429 (4)	428 (9)	418 (10)	414 (13)^ab	<0.01	0.352	0.627
DA	3.52 (1.49)	3.72 (1.15)	4.08 (1.52)	3.73 (1.36)	0.575	0.883	0.587

Values are mean (SD) and p < 0.05 was considered as significant. (C = control diet, CR = control diet + voluntary running, HF = 60% fat diet, HFR = 60% fat diet + voluntary running)
^ap < 0.05 vs C; ^bp < 0.05 vs CR; ^cp < 0.05 vs HF

ISSN: 1743-7075