Figure 3

Changes in intracellular free calcium concentration ([Ca²⁺] _i ). (A) Effect of GA on ([Ca²⁺]_i). [Ca²⁺]_i was measured under the G3 condition during the first 5 min, then under the G15 condition for the next 10 min, in the presence of 0.1 mg/mL HA (thick line) or GA (thin line). Data represent the mean plus SEM of 2 separate experiments, each with n = 4-10.. (B, C) [Ca²⁺]_i was measured in the presence of HA (thick line) or GA (thin line) under the G15 condition. Islet cells were incubated for the first 5 min, and then in the presence of 30 mM K⁺ (B) or 100 μM Tolb (C) for the next 10 min, in the presence of 0.1 mg/mL HA (solid) or GA (dotted). Data represent the mean plus SEM of 2 separate experiments, each with n = 4-10. ss; statistically significant.