Substrateb | Tissue | State 4 | State 3 | RCI |
---|
Pyruvate + CINN | Brain | 3.42 ± 0.33 | 5.88 ± 0.72 | 1.72 ± 0.13 |
 | Liver | 5.16 ± 0.19 | 10.08 ± 0.74 | 1.97 ± 0.21 |
LLA + CINN | Brain | 2.87 ± 0.04 | 3.83 ± 0.52 | 1.34 ± 0.20 |
 | Liver | 2.89 ± 0.29 | 3.68 ± 0.15 | 1.32 ± 0.20 |
DLA + CINN | Brain | 2.46 ± 0.29 | 2.79 ± 0.23 | 1.16 ± 0.23 |
 | Liver | 2.50 ± 0.40 | 2.52 ± 0.16 | 1.03 ± 0.10 |
- aRespiratory rates in nmol O2/mg mitochondrial protein per min.
- bSubstrate concentrations: pyruvate (10 mM + 2.5 mM malate) and LLA or DLA (5 mM + 2.5 mM malate); CINN, α-cyano-4-hydroxycinnamate (5 mM).
- State 4: The respiration state without ADP.
- State 3: The respiration state with the addition of ADP.
- RCR, Respiratory Control Ratio (state 3 rate:state 4 rate).
- ADP/O ratio: The ratio between the amount of ADP phosphorylated and oxygen consumed.
- *Significantly different from the values without substrate. Values were compared using one way ANOVA with LSD as post hoc test. α < 0.05
- Note: Refer to Table 1 and 2 for the respiratory parameters without substrates or with cocktail.