Table 1 qPCR primers used to assess gene expression of markers of lipid/fatty acid metabolism
Gene | Primer | Sequence 5′ _ 3′ | Accession numbera (Probe number) |
|---|---|---|---|
Chrebp | Forward Reverse | AATCCCAGCCCCTACACC CTGGGAGGAGCCAATGTG | NM_133552.1 (10) |
D5d | Forward Reverse | GAACTCTCTTCTGATTGGAGAGCTA CCGGAATTCATCAGTGAGC | AB052085.1 (26) |
D6d | Forward Reverse | AATTTCCAGATTGAGCACCAC AGTGGGGCAATCTTGTGC | NM_031344.2 (60) |
D9d | Forward Reverse | GAAGCGAGCAACCGACAG GGTGGTCGTGTAGGAACTGG | NM_139192.2 (125) |
Elovl2 | Forward Reverse | AACCTCGGAATCACACTTCTTT TCCCAGCTGGAGAGAACG | NM_001109118.1 (22) |
Elovl5 | Forward Reverse | TCGATGCGTCACTCAGTACC CCTTTGACTCGTGTGTCTCG | NM_134382.1 (122) |
Elovl6 | Forward Reverse | ATGGATGCAGGAAAACTGGA GCCCGCTTGTTCATCAGA | NM_134383.2 (119) |
Fasn | Forward Reverse | GGCCACCTCAGTCCTGTTAT AGGGTCCAGCTAGAGGGTACA | NM_017332.1 (6) |
Lxrα | Forward Reverse | CAGGAAGAGATGTCCTTGTGG TCTTCCACAACTCCGTTGC | NM_031627.2 (2) |
Lxrβ | Forward Reverse | AGCTCTGCCTACATCGTGGT GACCCTTCTTCCGCTTGC | NM_031626.1 (106) |
Srebf | Forward Reverse | ACAAGATTGTGGAGCTCAAGG TGCGCAAGACAGCAGATTTA | NM_001276707.1 (77) |