Dietary green-plant thylakoids decrease gastric emptying and gut transit, promote changes in the gut microbial flora, but does not cause steatorrhea

Table 1 Information about the primers used for determination of 16S rRNA genes by qPCR

Target bacterial group	Primer	Sequence (5′-3′)	Amplicon size (bp)	Annealing and elongation time (s)	Reference
Total bacteria	F-tot	GCA GGC CTA ACA CAT GCA AGT C	292	15	[39]
Total bacteria	R-tot	CTG CTG CCT CCC GTA GGA GT	292	15	[39]
Akkermansia muciniphilia-like bacteria	AM1-F	CAG CAC GTG AAG GTG GGG AC	327	20	[40]
Akkermansia muciniphilia-like bacteria	AM2-R	CCT TGC GGT TGG CTT CAG AT	327	20	[40]
Bacteroides fragilis group	Bfra-F	ATA GCC TTT CGA AAG RAA GAT	495	30	[41]
Bacteroides fragilis group	Bfra-R	CCA GTA TCA ACT GCA ATT TTA	495	30	[41]
Clostridium coccoides group	CcocF	AAA TGA CGG TAC CTG ACT AA	440	20	[41]
Clostridium coccoides group	CcocR	CTT TGA GTT TCA TTC TTG CGA A	440	20	[41]
Clostridium leptum subgroup	CleptF	GCA CAA GCA GTG GAG T	239	20	[42]
Clostridium leptum subgroup	CleptR3	CTT CCT CCG TTT TGT CAA	239	20	[42]
Enterobacteriaecae	Eco1457-F	CAT TGA CGT TAC CCG CAG AAG AAG C	195	10	[43]
Enterobacteriaecae	Eco1652-R	CTC TAC GAG ACT CAA GCT TGC	195	10	[43]
Lactobacillus	Lact-F	AGC AGT AGG GAA TCT TCC A	341	20	[44]
Lactobacillus	Lact-R	CAC CGC TAC ACA TGG AG	341	20	[45]

ISSN: 1743-7075