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Table 3 Assessment of the bias of 25(OH)D2 detected by LC-MS/MS using NIST certified SRM-972a

From: Determination of serum 25-hydroxyvitamin D status among population in southern China by a high accuracy LC-MS/MS method traced to reference measurement procedure

SRM-972a

25(OH)D2

Certified values by NIST

LC-MS/MS

Target values (ng/mL)

Uncertainty

Detected values (ng/mL)

SD (n = 3)

Bias(%)

Level 1

0.50

0.06

0.48

0.07

−4.17

Level 2

0.81

0.06

0.84

0.05

4.45

Level 3

12.90

0.30

12.72

0.26

−1.40

Level 4

0.50

0.10

0.51

0.04

2.26

  1. SRM standard reference material, NIST National Institute of Standards & Technology
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Nutrition & Metabolism

ISSN: 1743-7075

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