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Fig. 1 | Nutrition & Metabolism

Fig. 1

From: The long noncoding RNA MALAT1 modulates adipose loss in cancer-associated cachexia by suppressing adipogenesis through PPAR-γ

Fig. 1

The expression of MALAT1 in adipocyte. a Heatmap of four lncRNAs differentially expressed in subcutaneous WAT between CAC mice and control mice. The color intensity represents the normalized intensity (n = 6). b RNA FISH of MALAT1 (red) in C3H10 cells. DAPI was used as counterstaining (scale bar, 10 μm). c qPCR analyses of nuclear (Nuc) and cytoplasmic (Cyt) fractions of differentiated C3H10 cells. 12S rRNA served as a positive control in Cyt; 45S rRNA served as a positive control in Nuc. d, e Expression of adipogenic marker genes during adipogenesis of C3H10 cells as assessed by qPCR and immunoblotting. f Expression of MALAT1 during adipogenesis of C3H10 cells as assessed by qPCR. The experiments were repeated 3 times. The data represent means ± SEM. n = 3

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Nutrition & Metabolism

ISSN: 1743-7075

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