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Fig. 3 | Nutrition & Metabolism

Fig. 3

From: The deletion of glucagon-like peptide-1 receptors expressing neurons in the dorsomedial hypothalamic nucleus disrupts the diurnal feeding pattern and induces hyperphagia and obesity

Fig. 3

SCN projecting DMH neurons with GLP-1R expression increase c-Fos after feeding. A The injection site of the retrograde tracer, CTB in the SCN. Scale bar = 100 μm. B The distribution of CTB-positive neurons in the DMH. C The distribution of GLP-1R-positive neurons in the DMH. D A merged image of B and C. Scale bars = 50 μm. The enlarged images located in the left corner in the image of B–D indicate the image in the dotted white square in the respective image. Scale bars = 10 μm. Arrowheads indicate the neurons co-localizing CTB and GLP-1R. E–J: The distribution of CTB-positive neurons (E, H), c-Fos-positive neurons (F, I) and a merged image (G, J) in the DMH of a control fasting (E–G) or refeeding (H–J) rat. Scale bars in E–J indicate 10 μm. K The percentage of c-Fos-positive neurons among CTB-positive neurons in the DMH. (n = 5, 4) ** P < 0.01, Student t-test

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Nutrition & Metabolism

ISSN: 1743-7075

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