Fig. 4

Effects of estradiol on enter puberty and neurodevelopment in mice. a Body weight, wet carcass and dry carcass weights in E2 and CON group. b, c The weight of ovaries was no difference, while uterus weight was higher in E2 group than CON group. d Time spent in the open arms of the elevated plus maze was significantly increased in E2 as compared with CON mice. e Entries of food arm was increased of E2 than CON group in y-maze test. f, g Quantification of the levels of protein to kisspeptin1 and kisspeptin1 receptor in hypothalamus by western blotting. h Quantification of the levels of protein to NeuN, BDNF, TH and AVP in hypothalamus by western blotting, TH and AVP were higher in E2 group. Data are means ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001