Fig. 4

l-Arabinose promotes bile acid synthesis in the liver. The hepatic mRNA expressions of CYP7A1, CYP27A1, CYP7B1, and CYP8B1 (A) in HFHSD-fed mice treated with or without l-arabinose for 12 weeks. The protein levels of CYP7A1, CYP27A1, and CYP7B1 in the liver (B) with densitometric quantification (C). HSP90 was used as an internal reference. The hepatic mRNA expressions of FXR, SHP, HNF-4α, and BSEP (D). The protein expression of hepatic FXR and HNF-4α (E) with densitometric quantification (F).GAPDH was used as an internal reference. Data are shown as mean ± SEM. ^#p < 0.05 compared with the control group; ^##p < 0.01 compared with the control group; *p < 0.05 compared with the HFHSD-fed group; **p < 0.01 compared with the HFHSD-fed group. Ctrl control group, HFHSD HFHSD-fed group, HFHSD + L-Ara HFHSD-fed group treated with l-arabinose