Table 1 All primers for RT-PCR assay
Gene | Â | Primers |
|---|---|---|
MuRF1 | Forward | 5′-CCAGGTGAAGGAGGAACT-3′ |
Reverse | 5′-TTGGCACTCAAGAGGAAGG-3′ | |
MAFbx | Forward | 5′-CTTGTGCGATGTTACCCA-3′ |
Reverse | 5′-GTGAAAGTGAGACGGAGC-3′ | |
POMC | Forward | 5′-CCTCCTGCTTCAGACCTCCA-3′ |
Reverse | 5′-GGCTGTTCATCTCCGTTGC-3′ | |
AgRP | Forward | 5′-TGAAGGGCATCAGAAGGT-3′ |
Reverse | 5′-CACAGGTCGCAGCAAGGT-3′ | |
CART | Forward | 5′-GAAGTACGGCCAAGTCCCCA-3′ |
Reverse | 5′-AAGAAGTTCCTCGGGGACAGT-3′ | |
NPY | Forward | 5′-GTGTTTGGGCATTCTGGCTG-3′ |
Reverse | 5′-AGTGTCTCAGGGCTGGATCT-3′ | |
ghrelin | Forward | 5′-TGGTGTCTTCAGCGACTATCTGC-3′ |
Reverse | 5′-TCTCTGCTGGGCTTTCTGGTG-3′ | |
GHS-R1α | Forward | 5′-GTGTCCAGCGTCTTCTTCTTTC-3′ |
Reverse | 5′-GTGTCCAGCGTCTTCTTCTTTC-3′ | |
MUC2 | Forward | 5′-GCTGAACACTGGTGCTCCCTAC-3′ |
Reverse | 5′-GTCCTCGTTGTTCTGACAGTTGC-3′ | |
ZO-1 | Forward | 5′-CGGGCTACCTTATTGAATGTCC-3′ |
Reverse | 5′-GAGCGAACTGAATGGTCTGATG-3′ | |
β-actin | Forward | 5′-TGCTATGTTGCCCTAGACTTCG-3′ |
Reverse | 5′-GTTGGCATAGAGGTCTTTACGG-3′ | |
GAPDH | Forward | 5′-CTGGAGAAACCTGCCAAGTATG-3′ |
Reverse | 5′-GGTGGAAGAATGGGAGTTGCT-3′ |