Table 1 Primers sequences used for real-time quantitative PCR gene expression analysis
From: Curcumin improves insulin sensitivity in high-fat diet-fed mice through gut microbiota
Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
Pck1 | CTGCATAACGGTCTGGACTTC | CAGCAACTGCCCGTACTCC |
G6pc | CGACTCGCTATCTCCAAGTGA | GTTGAACCAGTCTCCGACCA |
Srebf1 | TGCGGCTGTTGTCTACCATA | TGCTGGAGCTGACAGAGAAA |
Fasn | TATCAAGGAGGCCCATTTTGC | TGTTTCCACTTCTAAACCATGCT |
Acaca | ATGGGCGGAATGGTCTCTTTC | TGGGGACCTTGTCTTCATCAT |
Scd1 | TTCTTGCGATACACTCTGGTGC | CGGGATTGAATGTTCTTGTCGT |
Fgf15 | ATGGCGAGAAAGTGGAACGG | CTGACACAGACTGGGATTGCT |
Rps18 | TTCCAGCACATTTTGCGAGTA | CACGCCCTTAATGGCAGTGAT |