Fig. 2

Validation of TOIDC as a novel small compound to reduce lipid accumulation. A Structure of TOIDC. B Representative Nile Red stained images of the vehicle and TOIDC-treated primary hepatocytes. Scale bar, 100 µm. C Quantification of (B). D IC50 curve of TOIDC in primary hepatocytes treated with oleic acid and palmitic acid. E Dose response of triglyceride content in the treated primary hepatocytes. F CCK8 assay of cell viability in TOIDC-treated primary hepatocytes. G Representative images of MitoTracker Red staining in primary hepatocytes treated with vehicle or TOIDC (15 µM) for 36 h. Scale bar, 50 µm. H Quantification of (G). Data are presented as mean ± SEM of three independent biological replicates; *P < 0.05; **P < 0.01; ns no significance