Fig. 7

Co-localization of PCSK9 with LDLR, CD36 and ANXA2. HepG2 cells were examined for PCSK9 secretion 16 and 40 h after serum depletion (A, n = 3). Cellular localization of PCSK9, LDLR, CD36 and ANXA2 proteins was examined by laser scanning confocal microscopy (B). Co-localization rate was used to quantify the degree of co-localization (C, n = 5). The scale bar has a width of 5 µm. Values are reported as mean ± SD. The p value was determined by unpaired T-test (A) or one-way ANOVA combined with Tukey’s multiple comparisons test (C). *p < 0.05, **p < 0.01, ***p < 0.001