Fig. 2

Plin5 deficiency had no effect on liver of mice fed a normal chow diet. A Representative western blot and quantification of Plin5 measured in livers of WT and Plin5 KO mice. B qPCR analysis of mRNA level of Plin5. C–E Body weight (C), liver mass (D) liver mass/body weight (E). F, G H&E and Masson staining images of liver sections from WT and Plin5 KO mice. Scale bar, 50 um. H, I Serum ALT and AST levels of mice in the indicated groups. J, K Serum TG (Triglyceride) and TC (total cholesterol) concentration in WT and Plin5 KO mice. L qPCR analysis of mRNA level of inflammation-related genes in the liver of WT and Plin5 KO mice fed a ND diet. M Relative mRNA level of profibrotic genes in liver tissue from WT and Plin5KO mice. A two-tailed student t test was used for statistical analysis. *< 0.05, **< 0.01, n.s. not significant. All data are shown as mean ± SEM. Abbreviations: MCP-1: Monocyte Chemoattractant Protein-1; TNF-α: Tumor necrosis factor-α; Collagen α-I: collagen type I alpha1 chain; TIMP-1: TIMP metallopeptidase inhibitor 1. (n = 6/group)