Fig. 3

Systemic knockout of Plin5 aggravated HFHC diet-induced steatohepatitis. A Schematic diagram of the experimental procedure used to examine the harmful role of Plin5 in mice fed an HFHC for 24 weeks. B–D Body weight (B), Liver mass (C), and liver mass/body weight (D) values in WT and Plin5 KO mice after feeding an HFHC diet for 24 weeks. E, F Serum TG and TC level. G Representative images of H&E and oil red O staining of liver sections from mice fed chow diet or HFHC diet. The statistics of oil red O-positive areas are shown. Scale bar, 50 um. H Relative images and quantitative data of PSR staining in liver sections from WT and Plin5 KO mice (scale bars 50 um. I, J Serum ALT (I) and AST (J) were measured in WT and Plin5 KO mice after 24 weeks of HFHC diet feeding (n = 6/group). K NAFLD activity score. L qPCR analysis of mRNA level of inflammation-related genes in the liver of WT and Plin5 KO mice fed the HFHC diet. M Relative mRNA level of profibrotic genes in liver tissue from WT and Plin5 KO mice. A two-tailed Student t test was used for statistical analysis. The data are presented as the mean ± SEM. A significant difference between the WT-ND group and the Plin5 KO-HFHC. group. *< 0.05, **< 0.01; Abbreviations: NAS, NAFLD activity score. (n = 6/group)