Fig. 5

Hepatocyte-specific overexpression of Plin5 alleviated MCD-induced NASH and ferroptosis. A Representative western blot of Plin5 measured in livers of AAV-vector and AAV-Plin5 mice. B, C Serum level of ALT and AST measured in WT and Plin5 KO mice after 3 weeks of MCD feeding. D Representative images of H&E, oil red O staining, Masson staining and PSR staining of liver sections from mice and NAFLD activity scores (Scale bar,100 um). E, F qPCR analysis of mRNA level of inflammation-related genes and profibrotic genes in the liver of AAV-vector and AAV-Plin5 mice fed an HFHC diet. G, H MDA and SOD content were measured by malondialdehyde assay kit (TBA method) and total superoxide dismutase assay kit (hydroxylamine method) respectively. I Expression of hepatic iron metabolic genes Tfr1, Hamp1, Hamp2, Fth and Ftl were measured by qPCR from AAV-vector and AAV-Plin5 mice under MCD-diet condition. J Hepatic mRNA level of Hmox1, Ptgs2, and Nox2 were measured by qPCR in MCD-diet mice. K The protein level of GPX4 and GAPDH in AAV-vector and AAV-Plin5 mice fed an MCD-diet were detected by western blot. A two-tailed Student t-test was used for statistical analysis. Data are presented as the mean ± SEM. *< 0.05, **< 0.01. (n = 6/group)