Fig. 6

Plin5 KO aggravated RSL-3 induced ferroptosis that was reversed by 11-Dodecenoic acid, a downstream targeting molecular of Plin5. A Schematic of the experimental strategy used to identify the potential target metabolites of Plin5. in steatotic hepatocytes. B Volcano plot indicates different metabolites between the Plin5 KO group and the control group. C Concentration of 11-Dodecenoic acid in WT and KO groups. D Protein expression and quantification of Plin5 in SK-HEP1 cells infected with siPlin5. E Schematic diagram of cells experiment. F Cell viability was assayed by trypan blue staining. G SK-HEP1 cells with empty vector, Plin5 knockdown, and adding 11-DA were treated with RSL-3 for 12 h and cell states were captured by microscope. Dead cells were stained by propidium iodide (PI). Scale bar, 100 um