Fig. 1
High global m6A methylation change in NAFLD models. A comparison of the mice body size, the mice liver size (A) and the mice weight (B) between the control group (blue) and the HFD group (red). The mice in HFD group (n = 16) were feeding with high fat diet for 16 weeks, while the mice in the control group were feeding with normal chow diet (n = 15). HE staining (C) (scale bars, 20 μm) of livers between the HFD and control group. The IPITT (D) and the IPGTT (E) tests in the HFD and control mice, the concentration of the injected insulin and glucose is 0.65 U/kg and 2.0 g/kg, respectively. LC–MS analysis (F) of m6A level in HFD mice liver and steatosis HepG2 cells (induced by 1 mmol/L FFA). Dot blot analysis (G) in the mice liver and in the steatosis HepG2 cells incubated with m6A antibodies. HE staining (scale bars, 20 μm) and the dot blot analysis (H) in the liver samples from NAFLD patients (n = 3) or CD patients (n = 3)