Fig. 2

METTL3 increased in hepatic IR and interference of METTL3 attenuated IR induced by high fat. PT-qPCR analysis of all writers (Alkb5 and Fto) and erasers (Mettl14, Mettl3, Virilizer, Wtap) mRNA expression in livers from HFD mice (red) and control mice(black) (A). Representative western blot analysis of all writers and erasers expression in HepG2 cells between FFA group and Control group (A). Representative western blot analysis and quantification of Mettl3 expression in HepG2 cells between FFA group and NC group (C). Glucose consumption (D) and glucose uptake (E) analysis in HepG2 cells, it was divided into six groups, disposed with or without insulin; with or without FFA; with or without Mettl3 SiRNA. Representative western blot analysis and quantification of Glut2 expression in HepG2 cells disposed with insulin (10⁻⁷ mmol/L insulin for 4 h) between METTL3 SiRNA group and Control group (F)