Fig. 4

METTL3 upregulated CYP2B6 m⁶A modification and positively regulate CYP2B6 expression. Merip-qPCR analysis of the m⁶A level of CYP2B6 between the METTL3 down-expression group (infected with METTL3 SiRNA) and the METTL3 over-expression group (transfected with LV5 lentivirus) (A). RIP assay was performed using anti-METTL3 antibodies (B). RT-qPCR analysis and western blot analysis of CYP2B6 expression in HepG2 between the group of METTL3 down-expression (METTL3 SiRNA) and the group of METTL3 over-expression (METTL3 LV5 lentivirus) (C). Representative western blot analysis and quantification of CYP2B6 expression in HepG2 from FFA + METTL3^−/− group, NC + METTL3^−/− group and FFA group (D). Dot blot test in mice liver from HFD + STM2457 group, HFD group, HFD + 0.9% NaCL group and HFD + DMSO group (E). Both IPITT and IPGTT results showed the increased insulin sensitivity in STM2457 group (F/G). Representative western blot analysis and quantification of CYP2B6 expression in HepG2 from HFD + STM2457 group and control group (H)