Table 1 The synthetic sequence information of primers used for RT-PCR
Genes | Sequences |
|---|---|
Mouse Cyp2b10 | Forward: 5’-TCCTGACCAGTTCCTGGATG-3’ |
|  | Reverse: 5’-CTGGAGGATGGACGTAAAGAA-3’ |
Human CYP2B6 | Forward: 5’-TTCACGGTACACCTGGGAC-3’ |
|  | Reverse: 5’-ATCACACCATATCCCCGGAAG-3’ |
Mouse Mettl3 | Forward: 5’-CTGGGCACTTGGATTTAAGGAA-3’ |
|  | Reverse: 5’-TGAGAGGTGGTGTAGCAACTT-3’ |
Human METTL3 | Forward: 5’-TTGTCTCCAACCTTCCGTAGT-3’ |
|  | Reverse: 5’-CCAGATCAGAGAGGTGGTGTAG-3’ |
Mouse Mettl14 | Forward: 5’-CTGAGAGTGCGGATAGCATTG-3’ |
|  | Reverse: 5’-GAGCAGATGTATCATAGGAAGCC-3’ |
Human METTL14 | Forward: 5’-GAGTGTGTTTACGAAAATGGGGT-3’ |
|  | Reverse: 5’-CCGTCTGTGCTACGCTTCA-3’ |
Mouse Wtap | Forward: 5’-TAGACCCAGCGATCAACTTGA-3’ |
|  | Reverse: 5’-CCTGTTTGGCTATCAGGCGTA-3’ |
Human WTAP | Forward: 5’-CTTCCCAAGAAGGTTCGATTGA-3’ |
|  | Reverse: 5’-TCAGACTCTCTTAGGCCAGTTAC -3’ |
Mouse Virilizer | Forward: 5’-ATGTCATGGAAACTGCACCTC-3’ |
|  | Reverse: 5’-GAGTGCTGAAAACCAAACCCA-3’ |
Human VIRILIZER | Forward: 5’-TACTTTGAGCCCATTTCTCCTGA-3’ |
|  | Reverse: 5’-GGAATACTGTCTACTGTTCGTCG -3’ |
Mouse Alkbh5 | forward: 5’-CGCGGTCATCAACGACTACC -3’ |
|  | Reverse: 5’-ATGGGCTTGAACTGGAACTTG-3’ |
Human ALKBH5 | Forward: 5’-CGGCGAAGGCTACACTTACG-3’ |
|  | Reverse: 5’-CCACCAGCTTTTGGATCACCA -3’ |
Mouse Fto | Forward: 5’-TTCATGCTGGATGACCTCAATG-3’ |
|  | Reverse: 5’-GCCAACTGACAGCGTTCTAAG-3’ |
Human FTO | Forward: 5’-ACTTGGCTCCCTTATCTGACC-3’ |
|  | Reverse: 5’-TGTGCAGTGTGAGAAAGGCTT -3’ |
Human PPARγ | Forward: 5’-TACTGTAGGTTTCAGAAATGCC -3’ |
|  | Reverse: 5’-GTCAGCGGACTCTGGATTCAG-3’ |