Fig. 1

D-mannose decreased the protein level of IDH2. (A) Western blot analysis of IDH2 level in MDA-MB-231 cells and MCF-7 cells treated with or without D-mannose. (B) Western blot analysis of IDH2 level in MDA-MB-231 cells treated with 100 mM D-mannose for different time as indicated. (C) Western blot analysis of IDH2 level in MDA-MB-231 cells treated with different concentrations of D-mannose as indicated for 48 h. (D) qPCR analysis of IDH2 mRNA level in MDA-MB-231 cells and MCF-7 cells treated with or without D-mannose. Values are means ± SD from n = 3 independent experiments. Statistical differences were determined by t-test. ns, not significant. (E) Western blot analysis of IDH2 level in MDA-MB-231 cells and MCF-7 cells treated with or without D-mannose and WZB117.