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Fig. 2 | Nutrition & Metabolism

Fig. 2

From: D-mannose promotes the degradation of IDH2 through upregulation of RNF185 and suppresses breast cancer

Fig. 2

D-mannose promotes IDH2 ubiquitination and proteasomal degradation. (A) The half-life of IDH2 under D-mannose treatment was determined by CHX-chase assay in MDA-MB-231 cells. (B) Western blot analysis of IDH2 level in MDA-MB-231 cells treated with D-mannose in the absence or presence of proteasome inhibitor MG132, or lysosome inhibitor NH4Cl. (C) Western blot analysis of IDH2 ubiquitination level under D-mannose treatment in 293T cells with overexpressed Flag-IDH2. (D) Western blot analysis of IDH2 ubiquitination level under D-mannose treatment in MDA-MB-231 cells

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Nutrition & Metabolism

ISSN: 1743-7075

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