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Fig. 4 | Nutrition & Metabolism

Fig. 4

From: D-mannose promotes the degradation of IDH2 through upregulation of RNF185 and suppresses breast cancer

Fig. 4

D-mannose elevates the expression of RNF185. (A) Heatmap showed the top 10 upregulated and top 10 downregulated genes by D-mannose treatment. (B) Venn diagram showed the cross analysis of RNA-seq data and BioGRID data. (C) qPCR analysis of RNF185 mRNA level in MDA-MB-231 cells and MCF-7 cells treated with or without D-mannose. Values are means ± SD from n = 3 independent experiments. Statistical differences were determined by t-test. ** p < 0.01. (D) Western blot analysis of RNF185 protein level in MDA-MB-231 cells and MCF-7 cells treated with or without D-mannose. (E) KEGG enrichment analysis of upregulated pathways in D-mannose treatment cells. (F) qPCR analysis of RNF185 mRNA level in MDA-MB-231 cells and MCF-7 cells treated with or without D-mannose and Compound C. Values are means ± SD from n = 3 independent experiments. Statistical differences were determined by t-test. ** p < 0.01

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Nutrition & Metabolism

ISSN: 1743-7075

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