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Fig. 5 | Nutrition & Metabolism

Fig. 5

From: D-mannose promotes the degradation of IDH2 through upregulation of RNF185 and suppresses breast cancer

Fig. 5

RNF185 binds with and degrades IDH2 under D-mannose treatment. (A) The interaction between Flag-IDH2 and RNF185 in 293T cells was determined by co-IP and western blot. (B) qPCR analysis of RNF185 mRNA level in MDA-MB-231 cells transfected with control or siRNF185. Values are means ± SD from n = 3 independent experiments. Statistical differences were determined by t-test. ****p < 0.0001. (C)Western blot analysis of RNF185 and IDH2 protein levels in MDA-MB-231 cells transfected with control or siRNF185. (D)Western blot analysis of RNF185 and IDH2 protein levels in MDA-MB-231 cells transfected with control or siRNF185 and treated with or without D-mannose

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Nutrition & Metabolism

ISSN: 1743-7075

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