Fig. 6

Synergistic action between riboflavin deficiency and PA load in increasing lipid accumulation in HepG2 cells. The experimental cells were cultured in media containing various concentrations of riboflavin (0, 3, 12, 24 nM) in the presence or absence of PA (10 μM) for 96 h. A Cell viability was determined by CCK8 assay. B TG content in HepG2 cells was detected using commercial kit. C Oil red O staining of HepG2 cells (40× magnification). D Quantitative determination of TG accumulation in HepG2 cells by a colorimetric method. Data are shown as mean ± SD, and experiments were performed in triplicate. *P < 0.05 versus NC; Comparisons between various concentrations of riboflavin (0, 3, 12, 24 nM) in the presence of PA (10 μM) versus the absence of PA were statistically significant at #P < 0.05