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Fig. 9 | Nutrition & Metabolism

Fig. 9

From: Effects of riboflavin deficiency and high dietary fat on hepatic lipid accumulation: a synergetic action in the development of non-alcoholic fatty liver disease

Fig. 9

PPARγ gene silencing partly reverses disturbed lipid metabolism caused by riboflavin deficiency or/and PA load in HepG2 cells. HepG2 cells were cultured in the riboflavin free medium for 96 h in the presence or absence of PA (10 μM) after blockade of PPARγ expression with PPARγ small interfering RNA (siRNA). A PPARγ and GAPDH protein bands in HepG2 cells. B The histogram is the grey value analysis of the corresponding protein bands. C PPARγ mRNA expression in HepG2 cells. D Quantitative determination of lipid droplets in HepG2 cells. E Oil red O staining of HepG2 cells (40× magnification). F TG content in HepG2 cells were detected using a commercial kit. The protein levels of FAS G, ATGL H and CPT1 I in HepG2 cells were analyzed by ELISA assay. *P < 0.05 versus NC; Comparisons before and after PPARγ transfection were statistically significant at #P < 0.05

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Nutrition & Metabolism

ISSN: 1743-7075

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