Fig. 2

Trex1-deficient rats develop Type 1 diabetes. A Serum insulin levels were measured in 8-week-old WT (n = 8) and Trex1^−/− (n = 8) rats after fasting for 4 h. B Blood glucose levels during an intraperitoneal insulin tolerance test were monitored in WT (n = 9) and Trex1^−/− (n = 10) rats at 16 weeks of age. C Serum GADA and IAA levels were measured by Elisa in 16-week-old WT (n = 8) and Trex1^−/− (n = 8) rats. D Representative H&E staining images of pancreatic section from WT and Trex1^−/− rats. Yellow circles indicate islets. Scale bar, 100 μm. E Representative fluorescence images of pancreatic sections from WT and Trex1^−/− rats stained with insulin (green) and glucagon (red) antibodies, indicating β cells and α cells respectively. The cell nuclei were stained with Hoechst (blue). Scale bar, 100 μm. F Percentage of β cells and α cells in islets from WT (n = 3) and Trex1^−/− (n = 3) rats. 15 islets in each of the rats were analyzed. All data are represented as mean ± SEM; Each dot represents one independent biological replicate; Unpaired t test in A, (C IAA); Welch’s t test in (C GADA); Two-way ANOVA with Sidak correction in F