Fig. 4

Trex1 deficiency results in accumulation of ssDNA and elevated production of IFN-β in pancreas. A Serum from WT (n = 9) or Trex1^−/− (n = 12) rats were obtained for ELISA analysis of IFN-β concentration. B The mRNA levels of Rsad2, Isg15, Ifit1, Mx1 in pancreas from WT (n = 6) and Trex1^−/− (n = 8) rats were measured by qPCR. Normalized by the housekeeping gene Gapdh. C Representative fluorescence images of the pancreas from WT or Trex1^−/− rats stained with antibodies recognizing ssDNA (red) and insulin (green). Nuclei were stained with Hoechst (blue). Scale bar, 20 μm. D Fluorescence scoring of ssDNA⁺ islets cell from WT or Trex1^−/− rats were quantified. (n = 3 per group). 10 islets in each of the rats were analyzed. E Fold change of indicated ISGs transcripts from RNA-seq data in the pancreas of WT or Trex1^−/− rats. F Fold change of indicated pro-inflammatory cytokines genes transcripts from RNA-seq data in the pancreas of WT or Trex1^−/− rats. All data are represented as mean ± SEM; Each dot represents independent biological replicate; Welch’s t test in A, B; Mann Whitney test in D