Fig. 5

Trex1-deficient rats develop diabetic cataracts and diabetic nephropathy. A Representative slit-lamp photographs of lens from WT or Trex1^−/− rats. B Representative H&E images showing lens sections from WT or Trex1^−/− rats. Scale bars, 100 μm. C Representative slit-lamp photographs of lens from different groups of animals at 0, 3, 6 and 9 weeks after the onset of diabetes in Trex1^−/− rats. D The progression of diabetic cataracts in WT (n = 6) or Trex1^−/− (n = 4) rats was quantified. E The UACR levels were calculated by the ratio of urinary albumin and creatinine in Trex1^−/− rats at 8 and 12 weeks after the onset of diabetes (n = 6 per group). F The urea and creatinine levels in serum were quantified at 4 and 12 weeks after the onset of diabetes in Trex1^−/− rats (n = 5 in each condition). G, H Representative images of kidney sections from WT or Trex1^−/− rats stained by periodic acid–Schiff (PAS) G or Masson’s trichrome H. Scale bars, 20 μm. All data are represented as mean ± SEM; Each dot represents independent biological replicate; Repeated measure ANOVA with Sidak post hoc test in D; Mann Whitney test in E, (F creatinine 12 w); Unpaired t-test in (F urea, F creatinine 4 w and 12 w); Welch’s t test in (F creatinine 4 w)