Fig. 4
Effect of oxyresveratrol (OR) on RANKL-induced bone-differentiation and bone-resorption marker genes. (a) Effect of OR on the NFATc1 mRNA level during osteoclast differentiation of RAW 264.7 cells. RAW 264.7 cells were seeded onto 35-mm culture dishes at 2 × 106/mL. Data are percentages versus RANKL (*p < 0.05, **p < 0.01, n = 3). (b–g) Effect of RANKL and OR (1 μM, or 10 μM) on NFATc1 (b, c), cathepsin K (d, e), and TRAP (f, g) protein levels in RAW 264.7 cells by western blotting. β-actin was used as the loading control. Data are percentages versus RANKL (*p < 0.05, ***p < 0.001, n = 3)