Fig. 3

In the prevention study, glutamine-based treatment has protective effects on high-fat diet-induced liver injury. a Liver/body weight (n = 10). b Liver TG levels (n = 10). c Results of HE and ORO staining of liver sections. Blue arrows indicate hepatic steatosis. d Results of Sirius red and F4/80 staining of liver sections. e mRNA expression of lipid transport-related genes in liver tissue (n = 5). (f) mRNA expression of lipid synthesis-related genes (n = 5). g mRNA expression of lipolysis-related genes (n = 5). h,i Western blot analysis of proteins and densitometric quantification (n = 3).Values are shown as the mean ± SEM. * Significantly different from the SD group. # Significantly different from the HFD group. Significance: * or #, p < 0.05; ** or ##, p < 0.01; *** or ###, p < 0.001. TG, triglyceride; CHOL cholesterol; HE, hematoxylin and eosin; ORO, Oil Red O