Fig. 6

Glutamine-based treatment in the reversal study does not improve high-fat diet-induced liver injury. a Liver/body weight (n = 12). b Liver TG levels (n = 10). c Results of HE and ORO staining of liver sections. Blue arrows indicate hepatic steatosis. Results of Sirius red and F4/80 staining of liver sections. d mRNA expression of lipid transport-related genes in liver tissue (n = 6). e mRNA expression of lipid synthesis-related genes (n = 6). f mRNA expression of lipolysis-related genes (n = 6). g Liver MDA levels (n = 12). h mRNA expression of SOD (n = 6). i mRNA expression of Gpx1 (n = 6). j mRNA expression of Cat (n = 6).Values are shown as the mean ± SEM. * Significant difference compared to the HFD group. Significance: *, p < 0.05; **, p < 0.01; ***, p < 0.001. TG, triglyceride; HE, hematoxylin and eosin; ORO, Oil Red O