Thyroid status influence on adiponectin, acylation stimulating protein (ASP) and complement C3 in hyperthyroid and hypothyroid subjects
© Yu et al; licensee BioMed Central Ltd. 2006
Received: 08 November 2005
Accepted: 10 February 2006
Published: 10 February 2006
Thyroid abnormalities (hyperthyroid and hypothyroid) are accompanied by changes in intermediary metabolism including alterations in body weight, insulin resistance and lipid profile. The aims of this study were to examine plasma ASP, its precursor C3 and adiponectin in hyperthyroid and hypothyroid subjects as compared to controls.
A total of 99 subjects were recruited from endocrinology/out-patient clinics: 46 hyperthyroid subjects, 23 hypothyroid subjects and 30 control subjects. Subjects were evaluated for FT4, FT3, TSH, glucose, insulin, complete lipid profile and the adipokines: adiponectin, acylation stimulating protein (ASP) and complement C3.
Hyperthyroidism was associated with a 95% increase in adiponectin (p = 0.0002), a 47% decrease in C3 (p < 0.0001), no change in ASP and increased ASP/C3 ratio (p = 0.0012). Hypothyroidism was associated with a 31% increase in ASP (p = 0.008). Adiponectin and C3 correlated with FT3 (r = 0.383, p = 0.004 and r = -0.277, p = 0.007, respectively) and FT4 (r = 0.464, p = 0.003 and r = -0.225, p = 0.03, respectively). ASP correlated with TSH (r = 0.202, p = 0.04). Adiponectin did not correlate with either ASP or C3, only ASP and C3 correlated (r = -0.197, p = 0.05). Adiponectin was negatively correlated with BMI, total cholesterol and plasma triglyceride, while C3 was positively correlated with BMI and total cholesterol. Surprisingly, adiponectin was positively correlated with insulin (r = 0.293, p = 0.02) and HOMA-IR (r = 0.373, p = 0.003) while C3 was negatively correlated with glucose (r = -0.242, p = 0.022, insulin (r = -0.184, p = 0.05) and HOMA-IR.
These changes suggest that thyroid disease may be accompanied by changes in adipokines, which may contribute to the phenotype expressed.
Thyroid abnormalities (hyperthyroid and hypothyroid) are accompanied by changes in intermediary metabolism including alterations in body weight, insulin resistance and lipid profile (review [1, 2]. Specifically, hyperthyroidism is characterized by decreased body weight, increased insulin and glucose indicative of insulin resistance as well as decreases in plasma lipids such as plasma cholesterol and triglycerides. These changes are a consequence of the cellular effect of thyroid hormone on liver, muscle and adipose tissue metabolism, which leads to increased energy expenditure. Conversely, hypothyroidism is associated with decreased levels of triiodothyronine (T3), thyroxine (T4) and increased thyroid stimulating hormone (TSH). These changes lead to increased body weight with increased plasma lipids and lipoproteins. Interestingly, plasma triglyceride, LDL cholesterol and HDL cholesterol are all increased in hypothyroidism and decreased in hyperthyroidism [2, 3], disrupting the normal inverse relationship seen between triglyceride and HDL cholesterol. As many of these changes are associated with alterations in glucose and insulin metabolism, and alterations in adipose tissue, we were interested in assessing the effect on adipokine hormones.
Adiponectin is a recently described adipose tissue hormone with multiple functions (review ). Plasma adiponectin has been reported to correlate with plasma lipids, in particular HDL cholesterol (HDL-C) (positively) [5, 6], as well as to insulin resistance (negatively) with decreased plasma levels often reported in type 2 diabetes [7, 8]. Interestingly, adiponectin, although produced in fat tissue, decreases in obesity . Functional studies suggest that adiponectin administration can alter insulin sensitivity, but it can also mediate numerous anti-atherosclerotic vascular functions such as endothelial function, inhibition of smooth muscle cell proliferation and inhibition of macrophage conversion to foam cells (review ). Adiponectin has been suggested to increase fatty acid oxidation and alter energy expenditure [9, 10], although this function remains controversial .
In contrast to the proposed effects of adiponectin on increasing energy expenditure, acylation stimulating protein (ASP) enhances energy storage through increasing postprandial triglyceride clearance, adipose tissue fatty acid esterification, glucose uptake and decreasing hormone sensitive lipase mediated lipolysis (review ). In a number of studies, ASP has been demonstrated to be increased in obesity, diabetes and cardiovascular disease (review ), and while not exclusively produced by adipose tissue, ASP generation increases in the adipose tissue milieu postprandially. Plasma ASP levels correlate positively with body mass index, as well as with plasma lipids such as total cholesterol, LDL cholesterol and apolipoprotein B. ASP often correlates with non-esterified fatty acids (NEFA). Interestingly, in C3(-/-) mice that are deficient in ASP, there is a decrease in adipose tissue mass with a repartitioning of ingested food towards increased energy expenditure (review ).
ASP, identical to C3adesArg, is produced through the alternate complement pathway via cleavage of complement C3, which is produced in multiple tissue sites, including the liver and adipose tissue. The cleavage of C3 is initiated through the action of adipsin (complement factor D), which, like adiponectin, is produced primarily in adipose tissue [13, 14]. Independent of its role in generation of ASP, C3 appears to have direct links to insulin resistance and plasma lipids, an association that is specific to this complement protein [15, 16]. C3 is increased in diabetes and in patients with coronary disease. C3 correlates strongly with insulin resistance , and in a study by Muscari et al, was shown to be a stronger predictor of insulin resistance and myocardial infarction than traditional risk factors .
The aims of this study were to examine plasma ASP, its precursor C3, and adiponectin in hyperthyroid and hypothyroid subjects as compared to controls. We tested (1) whether plasma ASP, C3 or adiponectin concentrations are associated with thyroid status in patients and (2) whether the levels of plasma C3 directly correlate with concentrations of ASP across all groups.
Research design and methods
A total of 99 subjects were recruited from endocrinology and out-patient daily clinics at the Tongji Medical Centre, Tongji Hospital, Wuhan, Hubei, P.R. China. Participants ranged from 18 to 67 years old. All participants gave informed consent and the study was approved by Tongji Hospital Ethics Committee. Subjects with diabetes, liver disease, heart disease, infectious disease or any other known disease were excluded. All subjects were ambulatory and were studied as out-patients. No subjects were taking any medication or herbal remedies known to affect lipid metabolism. Control subjects were recruited during their evaluation for a regular medical check-up. All control subjects were non-obese and had T3, T4 and TSH values within normal ranges (see below).
All thyroid patients were evaluated at the time of diagnosis during their initial visit to the Endocrinology Clinic. Onset of symptoms was recent (within 2–3 months) and no patients were taking any thyroid medication or herbal remedies. Hyperthyroid subjects were primarily diagnosed with Graves disease, and presented with T3 and T4 above the normal ranges and TSH below the normal range (see below for ranges). Hypothyroid subjects were diagnosed with auto-immune disease, with biochemical parameters of T3 and T4 below normal ranges, and TSH above the normal range.
Fasting blood samples and OGTT
Blood samples were drawn after an overnight fast from an antecubital vein. Fasting plasma samples were used for measurement of all parameters. Blood was centrifuged and plasma aliquoted and frozen immediately for future analysis of lipids and proteins.
Blood glucose was determined by glucose-oxidase method (AVE-852 half-auto biochemical analyzer). Plasma NEFA concentration was determined by colorimetric enzymatic assay (WAKO Chemicals, Tokyo, Japan). Plasma triglycerides were measured by GPO-PAP method and total cholesterol was measured by COD-PAP method. Following precipitation of apoB containing lipoproteins, the concentration of HDL-cholesterol (HDL-C) was also measured by enzymatic colorimetric assay (BCR, Ai-Weihali autobiochemical analyzer). Inter and intra coefficient of variation (CV) for all parameters (except HDL) were <3%. For HDL, inter and intra CV were <5%.
FT3 (free T3), FT4 (free T4) and TSH were measured by automated chemiluminescence immunoassay (ACS-180, American Kang Ning Company). The intra assay and interassay coefficient of variation were 2% and 6% respectively, for both FT3 and FT4. The normal range within the clinical biochemistry laboratory for T3 is 3.95–6.47 pmol/L, for T4 the normal range is 11.99–21.00 pmol/L and for TSH the normal range is 0.27–4.2 mU/L. Insulin was measured by electrochemiluminescence immunoassay (Elecsys 1010, Roche Instrument Center AG). Plasma adiponectin concentration was measured by ELISA (B-Bridge International, Phoenix, AZ, USA). Complement C3 was measured by an immunoturbidimetric assay (Lin- Fei Co, P.R. China). Plasma ASP concentration was measured via a sandwich ELISA immunoassay as previously described in detail [17, 18]. For these last assays, (insulin, adiponectin and ASP) intra-assay CV was <4% and interassay CV was <8%.
Body mass index (BMI) was calculated as weight (kg) per height (m2). Insulin resistance index was calculated by homeostasis model assessment, HOMA-IR, as (fasting insulin IU/L) * (fasting glucose mmol/L) / 22.5 as previously reported by Matthews . LDL cholesterol (LDL-C) was calculated according to the Friedewald formula as LDL-C = (total cholesterol mmol/L) – (triglyceride mmol/L)/2.2 – (HDL-C mmol/L) .
Unless otherwise stated, data are given as means ± standard error of the mean (SEM). Statistical comparisons of among all groups were performed using one-way ANOVA analyses. Correlations were analyzed with Pearson correlation coefficient or forward stepwise multiple regression analysis where P < 0.05 was considered to be statistically significant for all analyses.
Clinical and laboratory data in subject groups
41.7 ± 2.0
34.9 ± 1.6*
39.4 ± 2.5
22.0 ± 0.4
21.2 ± 0.4†††
24.2 ± 0.61*
4.88 ± 0.11
5.47 ± 0.07***
5.08 ± 0.12†
34.0 ± 5.5
60.8 ± 4.9***
33.8 ± 2.8††
1.10 ± 0.20
1.86 ± 0.16**
1.02 ± 0.11††
17.4 ± 0.4
64.5 ± 8.4***
9.1 ± 0.6*†††
5.7 ± 0.1
13.3 ± 1.2***
3.2 ± 0.2**†††
2.1 ± 0.1
0.03 ± 0.02***
69.5 ± 21.1**†††
Fasting lipid profiles in subject groups
1.14 ± 0.12
0.73 ± 0.06**,†††
1.74 ± 0.09***
4.58 ± 0.15
2.72 ± 0.14***,†††
6.16 ± 0.24***
2.40 ± 0.16
1.41 ± 0.09***,†††
3.66 ± 0.18***
1.56 ± 0.06
0.99 ± 0.04***
1.74 ± 0.07†††
0.361 ± 0.03
0.617 ± 0.05***
0.425 ± 0.03†††
As shown in Table 3, all three adipose tissue hormones and insulin correlated significantly with indices of thyroid function. Specifically, adiponectin and C3 correlated with FT3 (r = 0.382, p = 0.002 and r = -0.278, p = 0.007, respectively) and FT4 (r = 0.464, p = 0.0001 and r = -0.225, p = 0.03, respectively). Insulin also correlated with both FT3 and FT4 (r = 0.389, p = 0.003 and r = 0.395, p = 0.003, respectively). ASP correlated with TSH (r = 0.202, p = 0.04). Adiponectin did not correlate with either ASP or C3, only ASP and C3 correlated (r = -0.197, p = 0.05).
Correlations between thyroid hormones, adipokines and plasma parameters
The salient findings in the present study are the association of hyperthyroidism with increased adiponectin, decreased C3 and increased ASP/C3 ratio, and of hypothyroidism with increased ASP. Further, these changes in adipokines are each associated with specific changes in glucose or lipid metabolism which reflect whole body changes in energy expenditure/energy storage as discussed below. Thus this study provides important new data in this area.
With respect to adiponectin, the increased levels in hyperthyroidism are consistent with the increased metabolic rate that is characteristic of these patients. Our results contrast with those of Iglesias , where no significant changes were found. However it is difficult to draw conclusions since the average levels of adiponectin in that study were greater in all groups with a large range in values obtained (based on reported s.e.m.), the immunoassays used were different from the present study, and the age of the subjects was older. A study by Santini et al also examined adiponectin in hyperthyroid/hypothyroid subjects and found no significant differences. However the subject size was small, and this may have confounded the data (the authors suggest this to explain the lack of correlation in their study between adiponectin and BMI) . In contrast with these two studies, Yaturu and colleagues did find that adiponectin levels were higher in the hyperthyroid state than in the hypothyroid state, in agreement with our study . Further, a study of 68 healthy subjects demonstrated a positive association between plasma adiponectin and thyroid hormones although hyperthyroid and hypothyroid subjects were not tested directly .
Interestingly, in our study, the hyperthyroid subjects are also insulin resistant, with increased insulin levels. While insulin resistance is usually associated with decreased adiponectin levels, at least in diabetics [4, 7, 8], in hyperthyroidism this inverse relationship appears to be lost, and there is a direct correlation of adiponectin with plasma insulin and HOMA-IR. This is also true of the adiponectin association with plasma lipids. While several studies in controls, diabetic subjects and patients with cardiovascular disease have demonstrated a positive correlation of adiponectin with HDL-C [5, 6, 25–30], this is not true in the hyper/hypo thyroid subjects. In fact adiponectin correlates inversely with total plasma cholesterol and triglycerides but not HDL-C. This likely relates to the disruption of the usual elevated triglyceride/low HDL cholesterol relationship, which is a feature of hyper/hypothyroidism [2, 3]. This association of increased adiponectin with decreased plasma lipids but increased NEFA is consistent with an implication of adiponectin in fatty acid metabolism. Enhanced release of NEFA from adipose stores, coupled to increased adiponectin may stimulate fatty acid oxidation in muscle and liver  with a comparable decrease in lipoprotein production . The result is an anti-atherogenic lipid profile, which may be mediated through both thyroid hormones as well as adiponectin. This raises some interesting questions/hypotheses as to the mechanism of increased adiponectin secretion from adipocytes in hyperthyroid subjects. Evidence for a role of thyroid hormones in the regulation of adiponectin expression is suggested in a recent study showing increased adiponectin in mice exposed to cold . On the other hand, insulin has also been reported to stimulate adiponectin secretion [32–34], and the increased levels in the hyperthyroid subjects may also act as a stimulus. These hypotheses, however, remain to be tested experimentally.
In contrast to adiponectin, C3 was markedly decreased in hyperthyroid subjects, while ASP was increased in hypothyroid patients as compared to CTL. Directionally, the differences between hyper- and hypo- thyroid patients were the same for ASP and its precursor C3, in that both C3 and ASP were greater in hypothyroid than in hyperthyroid. However, the correlation of C3 with lipid and glucose parameters was stronger than the correlations with ASP. Other than the requirement of C3 as precursor for ASP, there is no known role for C3 in glucose or lipid metabolism, notwithstanding the many studies that have documented correlations of C3 with glucose and lipid metabolism and insulin resistance (review . This raises the question: Is there a direct effect of thyroid hormones on C3 production? Thyroid hormones bind to TREs (thyroid response elements), which heterodimerize with RAR and RXR (retinoic acid response elements) [35, 36], the latter of which is known to regulate C3 expression , explaining the close correlation with C3. Alternately, do the changes in thyroid hormones increase C3 conversion to ASP or ASP clearance rates? How thyroid hormones affect this process remains to be seen.
While this is the first report of ASP in hypothyroidism, the increased plasma levels of ASP are certainly consistent with the increased BMI and lipids. As detailed elsewhere (review , increased ASP is associated with obesity, diabetes and increased fasting plasma cholesterol, triglyceride, apolipoprotein B and NEFA. Further, in oral fat load studies, a higher ASP is associated with both increased fasting triglyceride and a delay in triglyceride clearance, suggesting ASP resistance . While we can only speculate, this may also be true of hypothyroid subjects. True to its proposed metabolic function, the increased plasma ASP levels are indicative of a push towards NEFA storage rather than oxidation, towards decreased energy metabolism, and towards an increase rather than a decrease in BMI. Interestingly in this setting of increased lipids but normal glucose, only ASP is increased while insulin remains at normal levels. These results dovetail with the studies on C3(-/-) ASP deficient mice, which are deficient in ASP and manifest increased energy expenditure with decreased body fat [39–41], while in the present subjects a decrease in energy expenditure coupled with an increase in BMI are associated with increased plasma ASP.
While, as noted above, there is no information available on the direct effects of thyroid hormones on adiponectin, ASP or C3 synthesis or secretion, there is additional information on other cytokines in thyroid dysfunction. IL-6 and TNFα are both increased in Grave's disease (hyperthyroid state) [42, 43], however since these cytokines have been reported to decrease adiponectin  and increase C3 , this cannot explain the demonstrated increase in adiponectin and decrease in C3 in the hyperthyroid state in the present study. However there is no lack of information on the thyroid hormone mediated effects on adipose tissue in vitro and in vivo. Thyroid hormone increases adipose tissue lipolysis as measured by increased NEFA or glycerol release [46–48] and increased β adrenergic response [49–51]. Contrasting with the push for lipolysis, hyperthyroidism induces down regulation of adipose fatty acid synthase and lipoprotein lipase; most of these effects reversed in hypothyroidism [52–54].
In summary, adipokines likely play an important role in mediating energy partitioning towards either utilization or storage. In that respect, ASP and adiponectin appear to have opposite metabolic effects. In hyperthyroid subjects, adiponectin is increased in a state where there is increased fatty acid oxidation, whereas in hypothyroidism ASP is increased where there is a push towards energy storage. Thus the well documented effects of thyroid hormone stimulation of energy expenditure in human and animal studies may partly be achieved through enabling effects on adipokines.
acylation stimulating protein
body mass index
high density lipoprotein cholesterol
homeostasis model assessment of insulin resistance
low density lipoprotein cholesterol
non-esterified fatty acids
peroxisome proliferator-activated receptor γ
We acknowledge the contribution of the Laboratory of Endocrinology and the Laboratory of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology for supporting this work. All authors contributed to acquisition of data, analysis, interpretation and manuscript preparation. We thank Lin Feng, Feng Xiao and Jun Wei for technical assistance and for their help in recruiting the subjects. Ping Yin provided statistical advice. KC is supported by a Canada Research Chair in Adipose Tissue. This study was supported by a grant from CIHR (OPP69600) and the FRSQ-NSFC Quebec-China exchange program (to KC).
- Heimberg M, Olubadewo JO, Wilcox HG: Plasma lipoproteins and regulation of hepatic metabolism of fatty acids in altered thyroid states. Endocr Rev. 1985, 6: 590-607.View ArticleGoogle Scholar
- Pucci E, Chiovato L, Pinchera A: Thyroid and lipid metabolism. Int J Obesity Rel Metab Dis. 2000, 24: S109-S112. 10.1038/sj/ijo/0801292.View ArticleGoogle Scholar
- Diekman MJ, Anghelescu N, Endert E, Bakker O, Wiersinga WM: Changes in plasma low-density lipoprotein (LDL)- and high-density lipoprotein cholesterol in hypo- and hyperthyroid patients are related to changes in free thyroxine, not to polymorphisms in LDL receptor or cholesterol ester transfer protein genes. J Clin Endocrinol Metab. 2000, 85: 1857-1862. 10.1210/jc.85.5.1857.View ArticleGoogle Scholar
- Ukkola O, Santaniem M: Adiponectin: a link between excess adiposity and associated comorbidities?. J Mol Med. 2002, 80: 696-702. 10.1007/s00109-002-0378-7.View ArticleGoogle Scholar
- Zietz B, Herfarth H, Paul G, Ehling A, Muller-Ladner U, Scholmerich J: Adiponectin represents an independent cardiovascular risk factor predicting serum HDL-cholesterol levels in type 2 diabetes. FEBS Lett. 2003, 545: 103-104. 10.1016/S0014-5793(03)00568-4.View ArticleGoogle Scholar
- Valsamakis G, Chetty R, McTerman PG, Al-Daghri NM, Barnett AH, Kumar S: Fasting serum adiponectin concentration is reduced in Indo-Asian subjects and is related to HDL cholesterol. Diabetes Obes Metab. 2003, 5: 131-135. 10.1046/j.1463-1326.2003.00254.x.View ArticleGoogle Scholar
- Yatagai T, Nagasaka S, Taniguchi A, Fukushima M, Nakamura T, Kuroe A: Hypoadiponectinemia is associated with visceral fat accumulation and insulin resistance in Japanese men with type 2 diabetes mellitus. Metabolism. 2003, 52: 1274-1278. 10.1016/S0026-0495(03)00195-1.View ArticleGoogle Scholar
- Spranger J, Kroke A, Mohlig M, Bergmann MM, Ristow M, Boeing H: Adiponectin and protection against type 2 diabetes mellitus. Lancet. 2003, 361: 226-228. 10.1016/S0140-6736(03)12255-6.View ArticleGoogle Scholar
- Yamauchi T, Kamon J, Minokoshi Y, Ito Y, Waki H, Uchida S: Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase. Nat Med. 2002, 8: 1288-1295. 10.1038/nm788.View ArticleGoogle Scholar
- Havel PJ: Control of energy homeostasis and insulin action by adipocyte hormones: leptin, acylation stimulating protein, and adiponectin. Curr Opin Lipidol. 2002, 13: 51-59. 10.1097/00041433-200202000-00008.View ArticleGoogle Scholar
- Stefan N, Vozarova B, Funahashi T, Matsuzawa Y, Ravussin E, Weyer C: Plasma adiponectin levels are not associated with fat oxidation in humans. Obes Res. 2002, 10: 1016-1020.View ArticleGoogle Scholar
- Cianflone K, Xia Z, Chen LY: Critical review of Acylation Stimulating Protein physiology in humans and rodents. Biochim Biophys Acta. 2003, 1609: 127-143.View ArticleGoogle Scholar
- Cianflone K, Sniderman AD, Walsh MJ, Vu H, Gagnon J, Rodriguez MA: Purification and characterization of acylation stimulating protein. J Biol Chem. 1989, 264: 426-430.Google Scholar
- Baldo A, Sniderman AD, St-Luce S, Avramoglu RK, Maslowska M, Hoang B: The adipsin-acylation stimulating protein system and regulation of intracellular triglyceride synthesis. J Clin Invest. 1993, 92: 1543-1547.View ArticleGoogle Scholar
- Muscari A, Massarelli G, Bastagli L, Poggiopollini G, Tomassetti V, Volta U: Relationship between serum C3 levels and traditional risk factors for myocardial infarction. Acta Cardiology. 1998, 53: 345-354.Google Scholar
- Pomeroy C, Mitchell J, Eckert E, Raymond N, Crosby R, Dalmasso AP: Effect of body weight and calorie restriction on serum complement proteins, including Factor D/adipsin: studies in anorexia nervosa and obesity. Clin Exp Immunol. 1997, 108: 507-515. 10.1046/j.1365-2249.1997.3921287.x.View ArticleGoogle Scholar
- Saleh J, Summers LKM, Cianflone K, Fielding BA, Sniderman AD, Frayn KN: Coordinated release of acylation stimulating protein (ASP) and triacylglycerol clearance by human adipose tissue in vivo in the postprandial period. J Lipid Res. 1998, 39: 884-891.Google Scholar
- Kalant D, Phelis S, Fielding BA, Frayn KN, Cianflone K, Sniderman AD: Increased postprandial fatty acid trapping in subcutaneous adipose tissue in obese women. J Lipid Res. 2000, 41: 1963-1968.Google Scholar
- Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC: Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia. 1985, 28: 412-419. 10.1007/BF00280883.View ArticleGoogle Scholar
- Friedewald WT, Levy RI, Fredrickson DS: Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972, 18: 499-502.Google Scholar
- Iglesias P, Alvarez FP, Codoceo R, Diez JJ: Serum concentrations of adipocytokines in patients with hyperthyroidism and hypothyroidism before and after control of thyroid function. Clin Endocrinol (Oxf). 2003, 59: 621-629. 10.1046/j.1365-2265.2003.01897.x.View ArticleGoogle Scholar
- Santini F, Marsilli A, Mammoli C, Valeriano R, Scartabelli G, Pelosini C: Serum concentrations of adiponectin and leptin in patients with thyroid dysfunctions. J Endocrinol Invest. 2004, 27: RC7-View ArticleGoogle Scholar
- Yaturu S, Prado S, Grimes SR: Changes in adipocyte hormones leptin, resistin, and adiponectin in thyroid dysfunction. J Cell Biochem. 2004, 93: 491-6. 10.1002/jcb.20188.View ArticleGoogle Scholar
- Fernandez-Real JM, Lopez-Bermejo A, Casamitjana R, Ricart W: Novel interactions of adiponectin with the endocrine system and inflammatory parameters. J Clin Endocrinol Metab. 2003, 88: 2714-2718. 10.1210/jc.2002-021583.View ArticleGoogle Scholar
- Pellme F, Smith U, Funahashi T, Matsuzawa Y, Brekke H, Wiklund O: Circulating adiponectin levels are reduced in nonobese but insulin-resistant first-degree relatives of type 2 diabetic patients. Diabetes. 2003, 52: 1182-1186.View ArticleGoogle Scholar
- Tschritter O, Fritsche A, Thamer C, Haap M, Shirkavand F, Rahe S: Plasma adiponectin concentrations predict insulin sensitivity of both glucose and lipid metabolism. Diabetes. 2003, 52: 239-243.View ArticleGoogle Scholar
- Yang WS, Lee WJ, Funahashi T, Tanaka S, Matsuzawa Y, Chao CL: Plasma adiponectin levels in overweight and obese Asians. Obes Res. 2002, 10: 1104-1110.View ArticleGoogle Scholar
- Yamamoto Y, Hirose H, Saito I, Tomita M, Taniyama M, Matsubara K: Correlation of the adipocyte-derived protein adiponectin with insulin resistance index and serum high-density lipoprotein-cholesterol, independent of body mass index, in the Japanese population. Clin Sci (Lond). 2002, 103: 137-142.View ArticleGoogle Scholar
- Haque WA, Shimomura I, Matsuzawa Y, Garg A: Serum adiponectin and leptin levels in patients with lipodystrophies. J Clin Endocrinol Metab. 2002, 87: 2395-10.1210/jc.87.5.2395.View ArticleGoogle Scholar
- Yang WS, Lee WJ, Funahashi T, Tanaka S, Matsuzawa Y, Chao CL: Weight reduction increases plasma levels of an adipose-derived anti-inflammatory protein, adiponectin. J Clin Endocrinol Metab. 2001, 86: 3815-3819. 10.1210/jc.86.8.3815.View ArticleGoogle Scholar
- Yoda M, Nakano Y, Tobe T, Shioda S, Choi-Miura NH, Tomita M: Characterization of mouse GBP28 and its induction by exposure to cold. Int J Obesity Rel Metab Dis. 2001, 25: 75-83. 10.1038/sj.ijo.0801482.View ArticleGoogle Scholar
- Stefan N, Stumvoll M: Adiponectin – its role in metabolism and beyond. Horm Metab Res. 2002, 34: 469-474. 10.1055/s-2002-34785.View ArticleGoogle Scholar
- Halleux CM, Takahashi M, Delporte ML, Detry R, Funahashi T, Matsuzawa Y: Secretion of adiponectin and regulation of apM1 gene expression in human visceral adipose tissue. Biochem Biophys Res Commun. 2001, 288: 1102-1107. 10.1006/bbrc.2001.5904.View ArticleGoogle Scholar
- Viengchareun S, Zennaro MC, Pascual-Le Tallec L, Lombes M: Brown adipocytes are novel sites of expression and regulation of adiponectin and resistin. FEBS Lett. 2002, 532: 345-350. 10.1016/S0014-5793(02)03697-9.View ArticleGoogle Scholar
- Cheng SY: Multiple mechanisms for regulation of the transcriptional activity of thyroid hormone receptors. Rev Endocr Metab Disord. 2000, 1: 9-18. 10.1023/A:1010052101214.View ArticleGoogle Scholar
- Harvey CB, Williams GR: Mechanism of thyroid hormone action. Thyroid. 2002, 12: 441-446. 10.1089/105072502760143791.View ArticleGoogle Scholar
- Scantlebury T, Sniderman AD, Cianflone K: Retinoic acid regulation of Acylation Stimulating Protein (ASP) and complement C3 in human adipocytes. Biochem J. 2001, 356: 445-452. 10.1042/0264-6021:3560445.View ArticleGoogle Scholar
- Cianflone K, Zakarian R, Couillard C, Delplanque B, Despres JP, Sniderman AD: Fasting acylation stimulating protein is predictive of postprandial triglyceride clearance. J Lipid Res. 2004, 45: 124-131. 10.1194/jlr.M300214-JLR200.View ArticleGoogle Scholar
- Xia Z, Sniderman AD, Cianflone K: Acylation-stimulating Protein (ASP) deficiency induces obesity resistance and increased energy expenditure in ob/ob mice. J Biol Chem. 2002, 277: 45874-45879. 10.1074/jbc.M207281200.View ArticleGoogle Scholar
- Murray I, Havel PJ, Sniderman AD, Cianflone K: Reduced body weight, adipose tissue, and leptin levels despite increased energy intake in female mice lacking acylation-stimulating protein. Endocrinology. 2000, 141: 1041-1049. 10.1210/en.141.3.1041.Google Scholar
- Xia Z, Stanhope KL, Digitale E, Simion O-M, Chen LY, Havel PJ: ASP deficiency results in increased energy expenditure in mice. J Biol Chem. 2004, 279: 4051-4057. 10.1074/jbc.M311319200.View ArticleGoogle Scholar
- Celik I, Akalin S, Erbas T: Serum levels of interleukin 6 and tumor necrosis factor-alpha in hyperthyroid patients before and after propylthiouracil treatment. Eur J Endocrinol. 1995, 132: 668-672.View ArticleGoogle Scholar
- Salvi M, Pedrazzoni M, Girasole G, Giuliani N, Minelli R, Wall JR: Serum concentrations of proinflammatory cytokines in Graves' disease: effect of treatment, thyroid function, ophthalmopathy and cigarette smoking. Eur J Endocrinol. 2000, 143: 197-202. 10.1530/eje.0.1430197.View ArticleGoogle Scholar
- Bruun JM, Lihn AS, Verdich C, Pedersen SB, Toubro S, Astrup A: Regulation of adiponectin by adipose tissue-derived cytokines: in vivo and in vitro investigations in humans. Am J Physiol Endocrinol Metab. 2003, 285: E527-E533.View ArticleGoogle Scholar
- Kalant D, Maslowska M, Scantlebury T, Wang H, Cianflone K: Control of lipogenesis in adipose tissue and the role of Acylation Stimulating Protein. Canadian Journal of Diabetes. 2003, 27: 154-171.Google Scholar
- Riis AL, Gravholt CH, Djurhuus CB, Norrelung H, Jorgensen JO, Weeke J: Elevated regional lipolysis in hyperthyroidism. J Clin Endocrinol Metab. 2002, 87: 4747-4753. 10.1210/jc.2002-020174.View ArticleGoogle Scholar
- Fryer LG, Holness MJ, Sugden MC: Selective modification of insulin action in adipose tissue by hyperthyroidism. Endocrinology. 1997, 154: 513-522. 10.1677/joe.0.1540513.View ArticleGoogle Scholar
- Richelsen B, Sorensen NS: Alpha 2- and beta-adrenergic receptor binding and action in gluteal adipocytes from patients with hypothyroidism and hyperthyroidism. Metabolism. 1987, 36: 1031-1039. 10.1016/0026-0495(87)90022-9.View ArticleGoogle Scholar
- Germack R, Starzec A, Perret GY: Regulation of beta 1- and beta 3-adrenergic agonist-stimulated lipolytic response in hyperthyroid and hypothyroid rat white adipocytes. Br J Pharmacol. 2000, 129: 448-456. 10.1038/sj.bjp.0703008.View ArticleGoogle Scholar
- Wahrenberg H, Engfeldt P, Arner P, Wennlund A, Ostman J: Adrenergic regulation of lipolysis in human adipocytes: findings in hyper- and hypothyroidism. J Clin Endocrinol Metab. 1986, 63: 631-638.View ArticleGoogle Scholar
- Gong DW, He Y, Karas M, Reitman M: Uncoupling protein-3 is a mediator of thermogenesis regulated by thyroid hormone, beta3-adrenergic agonists, and leptin. J Biol Chem. 1997, 272: 24129-24132. 10.1074/jbc.272.39.24129.View ArticleGoogle Scholar
- Blennemann B, Moon YK, Freake HC: Tissue-specific regulation of fatty acid synthesis by thyroid hormone. Endocrinology. 1992, 130: 637-643. 10.1210/en.130.2.637.Google Scholar
- Kern PA, Ranganathan G, Yukht A, Ong JM, Davis RC: Translational regulation of lipoprotein lipase by thyroid hormone is via a cytoplasmic repressor that interacts with the 3' untranslated region. J Lipid Res. 1996, 37: 2332-2340.Google Scholar
- Saffari B, Ong JM, Kern PA: Regulation of adipose tissue lipoprotein lipase gene expression by thyroid hormone in rats. J Lipid Res. 1992, 33: 241-249.Google Scholar
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