Figure 2

Histological assessment of liver fibrosis in CCl4-treated rats and the effects of the combination of melatonin and folic acid on collagen deposition. The extent of matrix deposition (arrow) was measured by Masson’s trichrome staining of liver tissue sections from control animals (A: 400x), CCl4-treated rats (black arrow: collagen of the very dilated central vein; red arrow: the interlobular collagen deposition) (B: 200x), CCl4-treated rats to show the inflammatory cells (C: 400x) and the rats receiving melatonin and folic acid (black arrow: collagen of the central vein) (D: 400x).