Measurement of total glucose in cells and medium. Short-term (acute) or continuous (chronic) cellular exposure to 100 μM ARG in the presence or absence of 30 μM L-NAME or CMP-C resulted in increase in cellular glucose (A). A progressive increase of glucose in the cell medium was also observed during ARG continuous treatment of cells for 7 days or when co-incubated with 30 μM L-NAME or CMP-C (B). Cells co-incubated with 100 μM ARG and 30 μM CDB, had glucose levels in cells and in the medium at levels similar to control conditions. n = 6. *, represents significant variation between control and treatment groups at p < 0.05. §, represents variation in treatment groups from cells treated with 100 μM ARG, at p < 0.05.