Effect of Sal A on plasma homocysteine concentrations and hepatic homocysteine transsulfuration activity. Plasma homocysteine levels (A), and hepatic activities of homocysteine transsulfuration enzymes, CBS (B) and CSE (C), in the normal rats (control) and hyperlipidemic rats after 4-week Sal A treatment (1 mg/kg/day, ip). Bars represent means ± SEM, n = 6. (D) In vitro effect of Sal A on CBS activity: liver homogenate as source of CBS activity was incubated with Sal A at the dose of 0.1, 0.2, 0.5, 1, 2, 4, 10, 25, 50 μmol/L at 37°C for 15 min. Control represents the CBS activity without Sal A. Bars represent means ± SEM, n = 8. *p < 0.05 (vs. control group). **p < 0.01 (vs. control group). ***p < 0.001 (vs. control group). #p < 0.05 (vs. hyperlipidemia group). ##p < 0.01 (vs. hyperlipidemia group). ###p < 0.001 (vs. hyperlipidemia group).