Nutrition & Metabolism

Table 3 Concentrations of selected plasma oxylipins and relative tissue mRNA abundances for oxylipin-relevant targets in mice with pre-existing obesity

From: A diet containing a nonfat dry milk matrix significantly alters systemic oxylipins and the endocannabinoid 2-arachidonoylglycerol (2-AG) in diet-induced obese mice

	Cohort 2
	Control		HighCa		NFDM
Oxylipin (nM)	Mean	SEM	Mean	SEM	Mean	SEM
∑C18 Diols
9,10-DiHOME	2.782	0.1526^a	2.749	0.1174^a	2.183	0.07732^b
12,13-DiHOME	1.813	0.1115^ns	1.752	0.07892^ns	1.516	0.07118^ns
9,10-DiHODE	0.04739	0.01185^a,b	0.06502	0.0143^a	0.01457	0.009056^b
15,16-DiHODE	1.224	0.1558^a	1.192	0.07191^a	0.4152	0.04656^b
∑C20 Diols
8,9-DiHETrE	3.176	0.1345^a	2.531	0.1684^b	1.054	0.07053^c
5,6-DiHETrE	2.871	0.129^a	2.332	0.1745^a	0.7654	0.157^b
11,12-DiHETrE	0.753	0.04288^a	0.7909	0.0363^a	0.6191	0.03119^b
14,15-DiHETrE	0.9577	0.03963^a,b	1.05	0.0584^a	0.88	0.06521^b
14,15-DiHETE	0.9577	0.03963^a,b	1.05	0.0584^a	0.88	0.06521^b
17,18-DiHETE	1.691	0.08384^a,b	1.883	0.07553^a	1.56	0.03399^b
∑C22 Diols
19,20-DiHDoPE	2.711	0.2254^a,b	3.41	0.2376^a	2.242	0.2042^b
∑Epoxides
15(16)-EpODE	1.524	0.1816^a	1.501	0.1288^a	0.9132	0.07219^b
mRNA abundance (%)
*Adipose*
Cyp2j6	100	9.5^a	123.2	14.8^a	218.1	29.8^b
Ephx2	100	15.7^a,b	79.55	13.0^a	144.6	17.3^b
Leptin	100	151.3^ns	48.84	59.1^ns	15.4	18.7^ns
Trpv1	100	9.3^ns	99.7	9.1^ns	139.5	19.7^ns
*Liver*
Ephx2	100	6.1^ns	98.45	7.0^ns	123.0	10.9^ns

SEM - Standard Error of the Mean.
n = 10/diet treatment group; For mRNA abundance, expression values are calculated relative to control; transcript level for CONTROL mice was considered 100%.
Data were tested for normality using D’Agostino and Pearson’s omnibus test.
To test for diet treatment differences, normal data were assessed by one-way ANOVA with Tukey post-hoc test, non-normal data were assessed by Kruskal-Wallis.
Values with different superscript letter are significantly different (P < 0.05). ns - not significant.

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ISSN: 1743-7075

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