Figure 1From: Phosphatidic acid enhances mTOR signaling and resistance exercise induced hypertrophyThe effect of various lipids on the activation of mTOR signaling. C2C12 myoblasts were stimulated for 20 minutes with vehicle (Control), or 10-30 μM of soy-derived (S) phosphatidylserine (S-PS), phosphatidylinositol (S-PI), phosphatidyl-ethanolamine (S-PE), phosphatidylcholine (S-PC), PA (S-PA), lysophosphatidic acid (S-LPA), diacylglycerol (DAG), glycerol-3-phosphate (G3P), or egg-derived PA (E-PA). The samples were then subjected to Western blot analysis for p70 phosphorylated on the threonine 389 residue (p70-389) and total p70. The ratio of these signals was calculated and used as a marker of mTOR signaling. Values in the graphs represent the mean + SEM and were obtained from 2–3 independent experiments (n = 4–12/group). * Significantly different from control (p < 0.001). # Significantly different from E-PA within each respective dose (p < 0.001).Back to article page