Figure 3

Dual fluorescence images of ALDH1A1 mRNA with ED1 co-staining for the detection of macrophages. Dual fluorescence ISH and IHC was performed using rat ALDH1A1 antisense probe and ED1 antibody, a rat macrophage marker, respectively, without and with DAPI staining of nuclei, on sections of VAD rat liver. ED1 was detected in the periportal area. ALDH1A1 staining (green) is increased after treatment with LPS. Some of the cells expressing ALDH1A1 showed overlapping staining with ED1 (red). Yellow; merged signals from ALDH1A1 and ED1; purple, merged signals from ED1 and DAPI nuclear stain. Magnification: x100. The co-localization of ALDH1A1 and ED1 is further illustrated for LPS-treated VAS liver at the bottom of Figure 3, where higher power images are shown (magnification: x200).