Figure 2

Daily faecal output of neutral sterols and bile acids of pigs fed the experimental diets. Pigs received diets containing 23% casein or 23% lupin protein isolate for 4 weeks. (A) major neutral sterols cholesterol and coprostanol, and total neutral sterols; (B) minor neutral sterols cholestanol, cholestanone and coprostanone; (C) major bile acids lithocholic acid and hyodeoxycholic acid, and total bile acids; (D) minor bile acids chenodeoxycholic acid, iso- lithocholic acid and hyocholic acid. Faeces were collected quantitatively during the last 5 days of the experiment. Major and minor neutral sterols were analysed by GC-FID, major and minor bile acids were analysed by GC-MS. Values are means (n = 6) with standard deviations. *Significantly different from the casein group (P < 0.05, Student’s t- test).