Skip to main content

Table 2 Comparison of array results and RT-PCR results for selected genes. Gene expression percentages are reported relative to the control values. F/ WR: Fasting Weight Reduction.

From: Regulation of mouse hepatic genes in response to diet induced obesity, insulin resistance and fasting induced weight reduction

Genes

Assay

High Fat vs. Control

F/ WR vs. Control

IL6st

Array

154 ± 21%*

144 ± 21%*†

 

RT-PCR

167 ± 19%*

185 ± 15%*†

PTP4a2

Array

71 ± 4%*

89 ± 3%*

 

RT-PCR

75 ± 16%

94 ± 18%

RGS3

Array

35 ± 5%*

54 ± 8%*

 

RT-PCR

38 ± 9%*

59 ± 8%*

G6P

RT-PCR

476 ± 72%

769 ± 216%*

PCK1

RT-PCR

132 ± 28%

217 ± 80%*

Malic Enzyme

RT-PCR

9.1 ± 1.5%*

0.1 ± 0.1%*

  1. *Indicates that the measurements were significantly different from control values at P < 0.01.
  2. †Indicates that the measurements made on the micro array were significantly different from the RT-PCR measurement at P < 0.05.