Skip to main content

Advertisement

Table 2 The conversion of glucose, acetate and glutamate to CO2 and lipid by liver slices from PEPCK-C-/- and control mice.

From: Phosphoenolpyruvate carboxykinase and the critical role of cataplerosis in the control of hepatic metabolism

  CO2 Total Lipid
Substrates PEPCK-C +/+ (n) PEPCK-C -/- (n) PEPCK-C +/+ (n) PEPCK-C -/- (n)
Glucose-U- 14 C 0.92 ± 0.26 (8) 0.30 ± 0.04 (5) 0.153 ± 0.009 (3) 0.063 ± 0.002 (2)
Acetate-2- 14 C 12.36 ± 0.88 (4) 2.72 ± 0.20 (5) 0.097 ± 0.018 (7) 0.002 ± 0.000 (2)
Glutamate-5- 14 C 5.15 ± 0.65 (2) 1.75 ± 0.65 (2) 1.778 ± 0.091 (4) 0.983 ± 0.098 (3)
  1. The values are expressed as μmoles of substrate converted to product/hour/g liver and represent the mean ± SE of the mean for the number of determinations shown in parenthesis. Livers slices from two-day-old mice were incubated for 2 h in Krebs Ringer bicarbonate buffer, pH 7.4, containing substrates at the concentrations outlined in the Methods, and the rates of 14CO2 production and 14C incorporation into total lipid determined. The radioactivity was quantified using a liquid scintillation spectrometer.