Effect of AICAR on phospho-status of ACC, AMPK and AMPK enzymatic activity in AICAR treated STD and DIO treated mice. Total protein from epididymal fat content from 6 weeks AICAR treated STD and DIO treated mice was isolated. Immunoblot analysis for p-ACC, p-AMPKα, AMPKα1 and β actin was examined as described in Materials and Methods (a). The enzymatic activity of AMPKα1 was examined by immunoprecipitating using anti-AMPKα1 followed by assessing its activity using SAMS peptide as described in Material and methods (b). *** p < 0.001; ** p < 0.01 as compared to STD mice group. $ p < 0.05, as compared to DIO vehicle treated group.