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Table 5 Mean number of copies per cell of the mRNAs for the selected genes in liver and adipose tissue of overweight male rats subjected to food restriction

From: Semiquantitative RT-PCR measurement of gene expression in rat tissues including a correction for varying cell size and number

Gene name Control Restricted feeding
  Cycles cpc Cycles cpc
  Liver
60S acidic ribosomal protein P0 (62 bp) 20.1 ± 0.1 1157 ± 50 20.1 ± 0.1 801 ± 38*
Cyclophilin A 19.4 ± 0.1 1261 ± 44 19.6 ± 0.1 855 ± 13*
Carbohydrate-responsive element-binding protein 22.8 ± 0.1 94 ± 6 23.3 ± 0.1* 54 ± 3*
Acetyl-CoA carboxylase 1 23.4 ± 0.2 50 ± 3 24.6 ± 0.1* 19 ± 1*
Insulin receptor substrate 1 25.0 ± 0.2 13 ± 1 24.9 ± 0.2 12 ± 1
  Retroperitoneal WAT
60S acidic ribosomal protein P0 (62 bp) 19.7 ± 0.1 160 ± 18 19.7 ± 0.1 191 ± 29
Cyclophilin A 19.1 ± 0.1 193 ± 30 19.4 ± 0.1 149 ± 7
Carbohydrate-responsive element-binding protein 24.4 ± 0.3 4.3 ± 0.6 26.2 ± 0.4* 1.0 ± 0.2*
Type II iodothyronine deiodinase 27.0 ± 0.1 0.63 ± 0.10 28.1 ± 0.2* 0.29 ± 0.03*
Acetyl-CoA carboxylase 1 23.0 ± 0.2 7.6 ± 1.8 23.5 ± 0.6 6.8 ± 2.2
Insulin receptor substrate 1 26.6 ± 0.2 0.46 ± 0.05 27.9 ± 0.4* 0.15 ± 0.04*
  1. The data are the mean ± sem of 7 different animals; cpc = copies of the corresponding mRNA per cell. Statistical significance of the differences between groups (Student's t test) * = P < 0.05.