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Table 5 Mean number of copies per cell of the mRNAs for the selected genes in liver and adipose tissue of overweight male rats subjected to food restriction

From: Semiquantitative RT-PCR measurement of gene expression in rat tissues including a correction for varying cell size and number

Gene name

Control

Restricted feeding

 

Cycles

cpc

Cycles

cpc

 

Liver

60S acidic ribosomal protein P0 (62 bp)

20.1 ± 0.1

1157 ± 50

20.1 ± 0.1

801 ± 38*

Cyclophilin A

19.4 ± 0.1

1261 ± 44

19.6 ± 0.1

855 ± 13*

Carbohydrate-responsive element-binding protein

22.8 ± 0.1

94 ± 6

23.3 ± 0.1*

54 ± 3*

Acetyl-CoA carboxylase 1

23.4 ± 0.2

50 ± 3

24.6 ± 0.1*

19 ± 1*

Insulin receptor substrate 1

25.0 ± 0.2

13 ± 1

24.9 ± 0.2

12 ± 1

 

Retroperitoneal WAT

60S acidic ribosomal protein P0 (62 bp)

19.7 ± 0.1

160 ± 18

19.7 ± 0.1

191 ± 29

Cyclophilin A

19.1 ± 0.1

193 ± 30

19.4 ± 0.1

149 ± 7

Carbohydrate-responsive element-binding protein

24.4 ± 0.3

4.3 ± 0.6

26.2 ± 0.4*

1.0 ± 0.2*

Type II iodothyronine deiodinase

27.0 ± 0.1

0.63 ± 0.10

28.1 ± 0.2*

0.29 ± 0.03*

Acetyl-CoA carboxylase 1

23.0 ± 0.2

7.6 ± 1.8

23.5 ± 0.6

6.8 ± 2.2

Insulin receptor substrate 1

26.6 ± 0.2

0.46 ± 0.05

27.9 ± 0.4*

0.15 ± 0.04*

  1. The data are the mean ± sem of 7 different animals; cpc = copies of the corresponding mRNA per cell. Statistical significance of the differences between groups (Student's t test) * = P < 0.05.