Figure 3
Effect of siPPARα and FFA on glucose-stimulated insulin secretion. Glucose-stimulated insulin release by UCP2 KO (A) and WT islets (B) after treatment with either control medium (defatted BSA, 1%), palmitic acid (PA, 0.4 mM + BSA) or oleic acid (OA, 0.4 mM + BSA) for 24 h in the presence of transilent siRNA control vector (open bars, n = 6) or transilent siPPARα in UCP2 KO islets (closed bars, n = 6). Two-way ANOVA performed on insulin release in response to 2.8 or 16.5 mM glucose showed a significant difference among treatment groups (P < 0.0001). Results of Tukey-Kramer multiple comparison test at each treatment showed a significant difference between siC and si PPARα (*P < 0.05 for effect of 16.5 mM glucose compared with matched 2.8 mM glucose groups; # P < 0.01 for effect of si PPARα).